CaRPool-seq

Cas13 RNA Perturb-seq (CaRPool-seq) is a method for multiplexed combinatorial perturbation screening with multimodal single-cell readout.

CaRPool-seq uses CRISPR array processing Cas proteins (i.e. RfxCas13d) to process polycistronic CRISPR arrays into multiple mature crRNAs alongside a detectable barcode (bcgRNA). You can read more about CaRPool-seq in our recent publication:

Wessels HH^# Mendez-Mancilla A^#, Hao Y, Papalexi E, Mauck WM III, Lu L, Morris JA, Mimitou EP, Smibert P, Sanjana NE, Satija R. Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq. Nature Mathods (2022). doi:10.1038/s41592-022-01705-x