Sequencing

Sequencing of all CaRPool-seq sub-libraries can be performed on Illumina instruments using standard recipes following 10x Genomics Feature Barcoding recommendations.

We designed the barcoded guide RNAs (bcgRNAs) to be compatible with additional single-cell modalities (i.e. CITE-seq), and to ensure efficient sequencing. The full bcgRNA information will be recovered running: 28 cycles for read1, 8 cycles for index i7, and 15 cycles for read 2. These setting are compatible with standard 10x Genomics recipes.

For detailed information, please refer to our Library structure and sequencing page.