Protocols

The experimental workflow for CaRPool-seq is very similar to running Perturb-seq experiments. A full description of the workflow can be found in our manuscript.

Here, we provide detailed protocols for individual steps in a CaRPool-seq experiment:

• Clone guide RNA onligos into pre-digested guide RNA plasmids. protocol

• Arrayed virus production. protocol

• Pooled virus production. protocol

• bcgRNA library amplification in CaRPool-seq. protocol

• Generate monoclonal Cas13d cell lines. coming soon